The Flytrap Project

The FlyTrap Protein Trap Stocks

The original FlyTrap web database has retired after long service. All internet traffic to the original FlyTrap database now directs here. 

Below you will find information about the Project and lists of the FlyTrap stocks that are still available from stock centers.

If you need more information about the lines or specific FlyTrap images, please contact the Cooley lab with your questions: kathleen.ayers@yale.edu

The FlyTrap Protein Trapping Project

Drosophila proteins tagged with Green Flourescent Protein (GFP) were created by insertion into genes of an artificial exon encoding GFP flanked by splice acceptor (SA) and splice donor (SD) sequences so that expression of GFP relies on splicing into mature mRNAs and in-frame fusion. 

This scheme results in expression under the control of endogenous promoter and enhancer elements, allowing for visualization of normal expression patterns.

The original screen was developed in Bill Chia's lab and published in Morin et al. (2001, PNAS 98:15050). A joint project, initiated by Mike Buszczak and involving the Cooley lab at Yale University (Quiñones-Coello et al., 2007, Genetics 175(3): 1089) and the Spradling lab at the Carnegie Institution of Washington (Buszczak et al., 2007, Genetics 175(3): 1505) accelerated the screening process by employing automated embryo sorters to examine millions of Drosophila embryos. Individual GFP-positive embryos were collected and allowed to develop to adulthood. Stocks were established from individual adults and matings were done to remove the starting elements and transposase source. The insertion sites in the resulting lines were determined by sequencing DNA flanking the transposons. Many of the flanking sequences were determined by the Drosophila Gene Disruption Project under the direction of Roger Hoskins at the Lawrence Berkeley Laboratory.

Ovaries and other tissues were examined for cellular and subcellular patterns of GFP-fusion protein expression. The position of insertions relative to genes, and the expression patterns of GFP revealed that we recovered both the expected Protein Traps as well as Enhancer Traps.

Trap Types and Contact Information

Trap Types

Expression is under the control endogenous promoter and enhance elements, allowing for visualization of normal expression patterns. Drosophila proteins tagged with Green Flourescent Protein (GFP) were created by insertion into genes of an artificial exon encoding GFP flanked by splice acceptor (SA) and splice donor (SD) sequences so that expression of GFP relies on splicing into mature mRNAs and in-frame fusion. 

This PDF contains the project information on this page.
























































The Protein Trapping Team

Participating Labs:

Chia Lab

(lines)

William Chia, PhD

Temasek Lifesciences Laboratory

1 Research Link

National University of Singapore

Singapore, 117604

web page: http://www.tll.org.sg/wchia.asp

email: wchia@tll.org.sg

Cooley Lab

(lines, ovary images)

Lynn Cooley, PhD

Yale University School of Medicine

Department of Genetics

333 Cedar St

New Haven, CT 06520

web page: http://medicine.yale.edu/lab/cooley/index.aspx

email: lynn.cooley@yale.edu

Spradling Lab

(lines, sequence)

Allan C. Spradling, PhD

Carnegie Institute of Washington

Department of Embryology

3520 San Martin Drive

Baltimore, MD 21218

web page: http://www.hhmi.org/research/investigators/spradling.html

email: spradling@ciwemb.edu

Berkeley Drosophila

Genome Project

(BDGP)

(sequences)

Roger Hoskins, PhD

Department of Genome Biology

Life Sciences Division

Lawrence Berkeley National Laboratory

Berkeley, CA 94720

email: hoskins@fruitfly.org

Axelrod Lab

(pupal wing disk images)

Jeff Axelrod, PhD

Stanford University School of Medicine

Department of Pathology

Edwards Building, Room R226a

300 Pasteur Drive

Stanford, CA 94305-5324

web page: http://www.stanford.edu/group/axelrodlab/

email: jaxelrod@stanford.edu

Please cite the appropriate publications when you use a FlyTrap line in your research.

Morin, X., R. Daneman, M. Zavortnik and W. Chia (2001) A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc. Natl. Acad. Sci. USA 98: 15050-15055

Kelso, R. J., M. Buszczak, A. T. Quiñones, C. Castiblanco, S. Mazzalupo and L. Cooley (2004) Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Research 32: D418-420.

Buszczak, M., S. Paterno, D. Lighthouse, J. Bachman, J. Plank, S. Owen, A. Skora, T. Nystul, B. Ohlstein, A. Allen, J. Wilhelm, T. Murphy, R. Levis, E. Matunis, N. Srivali, R. Hoskins, and A.C. Spradling (2007) The Carnegie protein trap library: a versatile tool for Drosophila developmental studies. Genetics 175(3): 1505-1531.

Quiñones-Coello, A.T., L.N. Petrella, K. Ayers, A. Melillo, S. Mazzalupo, A.M. Hudson, S. Wang, C. Castiblanco, M. Buszczak, R.A. Hoskins, and L. Cooley (2007) Exploring strategies for protein trapping in Drosophila. Genetics 175(3): 1089-1104.

Where the lines came from:

Line Prefix

GFP reading frame used

Transposon

Lab of origin

Citation

BA

A

PBac

Spradling

Buszczak et al

CA

A

P element

Spradling

Buszczak et al

CB

B

P element

Spradling

Buszczak et al

CC

C

P element

Spradling

Buszczak et al

G

A, B, C

P element

Chia

Morin et al

P

A, B, C

P element

Cooley

Quiñones-Coello et al

YB

B

P element

Cooley

Quiñones-Coello et al

YC

C

P element

Cooley

Quiñones-Coello et al

YD

A

PBac

Cooley

Quiñones-Coello et al

ZCL

A, B, C

P element

Chia

Morin et al

FlyTrap Stocks

Many of the FlyTrap stocks from the screen are available from public stock centers, and many of the sequences are available on FlyBase.

This file contains a list of available FlyTrap stocks, with the corresponding stock centers where you can still find them. Insertion sites correspond to Genome release 5.

This link leads to a list of Protein Trap stocks at Bloomington (BDSC), which includes some of the FlyTrap lines.

This link leads to a list of Protein Trap stocks at Kyoto (DGRC), which includes many FlyTrap lines.